New product released for mouse on mouse immunofluorescence staining: MaxFluor Dyes

Mice have been used in biotechnology medical research since 1894. It is the most common experimental animal in laboratories worldwide. Mice is also been used widely to produce monoclonal primary antibodies. For cancer research pathology study, the researchers often encounter the challenge of staining mouse primary antibody on mouse tissue due to limited choices of primary antibodies other than mouse originated. The major problem is the inability of anti-mouse secondary antibody to distinguish between endogenous mouse immunoglobulins and mouse primary antibody using indirect method. This problem will cause high background staining while the specific staining is then obscured. Endogenous IgGs are mainly distributed in intercellular space and will cause particular problems for antigens localized near cell surface or in extracellular matrix. It is also very difficult for membrane antigens if using mouse primary antibody to detect the membrane antigens on cryostat sections without fixation. The level of background is tissue type-related and varies from sample to sample. High background has been the major challenge for the researchers who have to use mouse primary antibodies on mouse tissue in immunofluorescence staining.

MaxVision have released new products for Mouse on Mouse Immunofluorescence Staining ideal as a solution for this ongoing issue in many laboratories.


-----------------------------------------------------------------------------------------------
Features:

For immunofluorescence detection of mouse primary antibody on mouse tissue; Biotin-free technology


Key Benefits:

Super clean background
Significant increase in fluorescence signal
Minimum cross-reactivity to endogenous immunoglobulins with MaxMOM™ blocking reagent
Only pick up mouse primary antibody signal not endogenouse immunoglobulins
3-4 times more sensitive and cleaner background than direct method
No biotin blocking steps and completely elimination of biotin background
Best signal:noise ratio


Applications: Immunofluorescence

Reactivity: Detect mouse primary antibody on mouse tissue, can also be used on human tissue.

Applicable Tissue Type: Formalin Fixed Paraffin Embedded Section, Frozen tissue and cell culture preparations.


Kit major components:

Protein Block (ready to use)
MaxMOM™ Blocking Reagent (ready to use)
Fluorescence Signal Enhancer (ready to use)
MaxFluor™ Linker (concentrate)
Fluorescent Diluent



-----------------------------------------------------------------------------------------------
Background:

MaxFluor™ Mouse on Mouse Fluorescence Detection Kits is designed to localize mouse primary antibody on mouse tissue in immunofluorescence application. The major problem encountered by the investigators when they try staining mouse primary antibodies on mouse tissue using indirect method is the inability of anti-mouse secondary antibody to distinguish between endogenous mouse immunoglobulins and mouse primary antibody. This problem will cause high background staining while the specific staining is then obscured. MaxMOM™ blocking reagent is special designed and formulated to block endogenous immunoglobulins which lead to the most complete elimination of background staining. With the proprietary signal enhancer which contains background reducing agent, the user may expect the best signal-to-noise ratio using MaxFluor™ Mouse on Mouse Fluorescence Detection Kits.


-----------------------------------------------------------------------------------------------
Where can I find more information about MaxVision?

Visit the manufacturer page at www.stratech.co.uk/MaxVision, email [email protected] or call +44 (0) 1638 782600.

Stratech Scientific is a distributor of high quality, competitively priced, reliable products for research laboratories throughout the UK and Europe. Please contact us to find out which ranges we can supply in your country.

Please contact Dr. Luke Scicluna for all marketing related queries.

-----------------------------------------------------------------------------------------------